Through the proposed method it can be clearly known that a human being (specially a human baby) is suffering from sickle cell anaemia or not by a fast and efficient way. This disease is caused by mutation of the gene controlling Beta-chain of haemoglobin (Hb). It replaces Glutamic acid (GAG) present at 6th position of the Beta-chain by Valine (GTG). The mutant haemoglobin molecule undergoes polymerization under low Oxygen tension causing the change in the shape of the RBC from biconcave disc to elongated Sickle-like structure. With the help of this test, it can be known that a human is a Mutant of this disorder or not, which can save the life of new generation.

Beta chain of Haemoglobin, Glutamic acid, Valine

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